Journal: Biomolecules and Biomedicine
Article Title: UPP1 and AHSA1 as emerging biomarkers and targets in pancreatic cancer: A proteomic approach
doi: 10.17305/bb.2025.11958
Figure Lengend Snippet: GEPIA database (A), RT-qPCR (B), and Western blot (C) detected the expression of UPP1, AHSA1 and LACTB in PC vs para-PC group. * P < 0.05, *** P < 0.001, **** P < 0.0001. PC: Pancreatic cancer; UPP1: Uridine Phosphorylase 1; LACTB: Lactamase Beta; AHSA1: Activator of HSP90 ATPase Activity 1; RT-qPCR: Reverse transcription-quantitative polymerase chain reaction; GEPIA: Gene Expression Profiling Interactive Analysis.
Article Snippet: The primary antibodies used were UPP1 (Wuhan Sanying Biotechnology, 1:2000), LACTB (Boster, 1:2000), AHSA1 (Boster, 1:2000), and GAPDH (Affinity, 1:20,000).
Techniques: Quantitative RT-PCR, Western Blot, Expressing, Activity Assay, Reverse Transcription, Real-time Polymerase Chain Reaction, Gene Expression